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2984127 
Journal Article 
Optimization of RNA extraction from FFPE tissues for expression profiling in the DASL assay 
Abramovitz, M; Ordanic-Kodani, M; Wang, Y; Li, Z; Catzavelos, C; Bouzyk, M; Sledge, GW; Moreno, CS; Leyland-Jones, B 
2008 
Yes 
BioTechniques
ISSN: 0736-6205 
44 
3 
417-423 
English 
18361796 
Formalin-fixed paraffin-embedded (FFPE) breast tumor tissues are readily available and represent a largely untapped, vast resource for molecular profiling of clinical samples with long-term follow-up data. We have optimized the conditions and parameters that result in the preparation of total RNA that is of the necessary quality for use in the DASL (cDNA-mediated annealing, selection, extension, and ligation) assay in which expression of 502 genes are analyzed simultaneously using as little as 100 ng of input RNA.