Health & Environmental Research Online (HERO)


Print Feedback Export to File
3002781 
Journal Article 
Ultrasensitive UPLC-MS-MS Method for the Quantitation of Etheno-DNA Adducts in Human Urine 
Cui, S; Li, H; Wang, S; Jiang, X; Zhang, S; Zhang, R; Sun, Xin 
2014 
Yes 
International Journal of Environmental Research and Public Health
ISSN: 1661-7827
EISSN: 1660-4601 
11 
10 
10902-10914 
English 
25337939 
WOS:000344358700055 
Etheno-DNA adducts are generated from the metabolism of exogenous carcinogens and endogenous lipid peroxidation. We and others have previously reported that 1,N-6-ethenodeoxyadenosine (epsilon dA) and 3,N-4-ethenodeoxycytidine (epsilon dC) are present in human urine and can be utilized as biomarkers of oxidative stress. In this study, we report a new ultrasensitive UPLC-ESI-MS/MS method for the analysis of epsilon dA and epsilon dC in human urine, capable of detecting 0.5 fmol epsilon dA and 0.3 fmol epsilon dC in 1.0 mL of human urine, respectively. For validation of the method, 20 human urine samples were analyzed, and the results revealed that the mean levels of epsilon dA and epsilon dC (SD) fmol/mu mol creatinine are 5.82 +/- 2.11 (range 3.0-9.5) for epsilon dA and 791.4 +/- 328.8 (range 116.7-1264.9) for epsilon dC in occupational benzene-exposed workers and 2.10 +/- 1.32 (range 0.6-4.7) for epsilon dA and 161.8 +/- 200.9 (range 1.8-557.5) for epsilon dC in non-benzene-exposed workers, respectively. The ultrasensitive detection method is thus suitable for applications in human biomonitoring and molecular epidemiology studies. 
UPLC-MS/MS; 1,N-6-ethenodeoxyadenosine; 3,N-4-ethenodeoxycytidine; lipid peroxidation; human biomonitoring