US EPA

3015957 

Journal Article 

Comparative study of cytokinin production isolated from bacteria and shoot induction 

Sabat, S; Murthy, VK; Shantha, SL; Kushnoor, D; Agarwal, G; Thomas, J; Devaraj, S 

2014 

Yes 

Indian Journal of Biotechnology
ISSN: 0972-5849 

13 

4 

544-546 

WOS:000349813000018 

Rhizobium sp., isolated from Mimosa pudica, Bacillus subtilis BCl and Escherichia coli K12 were investigated for their ability to produce plant growth hormones in a given production media (PM1 & PM2). Thin layer chromatography and high pressure liquid chromatography were performed to analyse the amount of cytokinin produced by these microorganisms. E. coli K12 produced the highest amount (0.186 mg/L) of cytokinin in PM2 medium, while B. subtilis BCl gave the least production amount (0.0075 mg/L) in the same medium. The microbially synthesized cytokinin was then incorporated onto the solidified MS medium for the shoot development in Phillodendron xanadu, an ornamental plant. The cytokinin produced from E. coli K12 gave more number of plantlets with thick shoot and lustrous leaves having 3.0 cm length for medium with charcoal (PM2); while comparatively less number of plantlets having 1.5 cm length for medium without charcoal. The cytokinin from B. subtilis BCl and Rhizobium sp. gave comparatively more shoot number and more plant height on medium with charcoal in PM1 and PM2 respectively. 

Bacillus subtilis; cytokinin; Escherichia coli; Phillodendron xanadu; Rhizobium sp.; shoot induction