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3016464 
Journal Article 
Abstract 
IgE cross-reactivity between house dust mite and Chinese liver fluke antigens 
Kim, JY; Kim, TY; Kim, CR; Yi, MH; Yong, TS 
2015 
1 
Tropical Medicine and International Health
ISSN: 1360-2276
EISSN: 1365-3156 
20 
Supplement 1 
289 
English 
WOS:000360758801305 
is part of a larger document 3513636 Abstracts of the 9th European Congress on Tropical Medicine and International Health, 6-10 September 2015, Basel, Switzerland - Poster Sessions
Introduction: Identification of common allergens between parasites and house dust mites (HDMs) is important to understand modulation of allergic diseases. Clonorchiasis caused by infection of Chinese liver fluke Clonorchis sinensis is the most prevalent parasitic disease in South Korea. IgE cross-reactivity between HDM allergens and C. sinensis antigens was investigated in this study.

Materials and Methods: Human clonorchiasis (n = 31), opisthorchiasis (n = 37), HDM-sensitized allergic (n = 88) and normal (n = 23) sera prepared. To eliminate possible C. sinensis infection among the HDM-sensitized allergic subjects, IgG ELISA against C. sinensis crude lysate was primarily performed. IgE ELISA against C. sinensis crude lysate or two HDM (Dermatophagoides farinae and D. pteronyssinus) extracts was performed to investigate the cross-reactivity. Cross-reactive allergen was confirmed by IgE immunoblot.

Results: HDM-sensitized allergic subject exhibited 0.6% of reactivity to C. sinensis antigens. Among the HDM-sensitized allergic subjects, 83.3% exhibited reactivity with C. sinensis antigens by IgE ELISA. Clonorchiasis and opisthorchiasis subjects exhibited 58% and 18.9% of IgE cross-reactivity with D. farinae extract, respectively. Similar cross-reactivity was observed when D. pteronyssinus extract was used. IgE immunoblot showed multiple cross-reacting bands. Most of the bands appeared to have over 100 kDa of molecular masses.

Conclusions: The present study demonstrated the presence of multiple IgE cross-reacting allergens between C. sinensis and HDM. Individual identification of the allergens from C. sinensis may provide some clues to understand the modulation of allergic diseases by parasitic infections.

Disclosure: Nothing to disclose. 
9th European Congress on Tropical Medicine and International Health 
Basel, Switzerland 
September 6-10, 2015