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3041132 
Journal Article 
Use of human keratinocyte and fibroblast cultures for toxicity studies of topically applied compounds 
Ponec, M; Haverkort, M; Soei, YL; Kempenaar, J; Bodde, H 
1990 
Yes 
Journal of Pharmaceutical Sciences
ISSN: 0022-3549
EISSN: 1520-6017 
IPA/91/946012 
Pharm 
REF 27 
English 
IPA COPYRIGHT: ASHP The potential skin toxicity of ethyl alcohol (ethanol; I), propylene glycol (II), dimethyl sulfoxide (III), dimethylformamide (IV) and oleth 10 (Brij 96; V) was studied in cultured human keratinocytes and fibroblasts; the inhibition of the proliferation of fibroblasts and keratinocytes, inhibition of collagen contraction by fibroblasts and cell morphology changes in confluent cultures of fibroblasts and keratinocytes were examined. In all assays performed, I was the least toxic; II, III and IV were moderately potent; and V was the most toxic agent. Advantages of the model included its immediate availability and reproducibility. However, this single-cell model lacked some properties found in intact skin.