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3046004 
Journal Article 
Bioreduction of alpha,beta-unsaturated ketones and aldehydes by non-conventional yeast (NCY) whole-cells 
Goretti, M; Ponzoni, C; Caselli, E; Marchegiani, E; Cramarossa, MR; Turchetti, B; Forti, L; Buzzini, P 
2011 
Yes 
Bioresource Technology
ISSN: 0960-8524
EISSN: 1873-2976 
102 
5 
3993-3998 
English 
21232941 
WOS:000287786300001 
The bioreduction of alpha,beta-unsaturated ketones (ketoisophorone, 2-methyl- and 3-methyl-cyclopentenone) and aldehydes [(S)-(-)-perillaldehyde and alpha-methyl-cinnamaldehyde] by 23 "non-conventional" yeasts (NCYs) belonging to 21 species of the genera Candida, Cryptococcus, Debaryomyces, Hanseniaspora, Kazachstania, Kluyveromyces, Lindnera, Nakaseomyces, Vanderwaltozyma, and Wickerhamomyces was reported. The results highlight the potential of NCYs as whole-cell biocatalysts for selective biotransformation of electron-poor alkenes. A few NCYs exhibited extremely high (>90%) or even total ketoisophorone and 2-methyl-cyclopentenone bioconversion yields via asymmetric reduction of the conjugated C=C bond catalyzed by enoate reductases. Catalytic efficiency declined after switching from ketones to aldehydes. High chemoselectivity due to low competing carbonyl reductases was also sometimes observed. (C) 2010 Elsevier Ltd. All rights reserved. 
Non-conventional yeasts; alpha,beta-Unsaturated ketones; alpha,beta-Unsaturated aldehydes; Bioreduction; Enoate reductase