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3046698 
Journal Article 
VALIDATION OF REFLECTANCE INFRARED-SPECTROSCOPY AS A QUANTITATIVE METHOD TO MEASURE PERCUTANEOUS-ABSORPTION IN-VIVO 
Higo, N; Naik, A; Bommannan, DB; Potts, RO; Guy, RH 
1993 
1 
Pharmaceutical Research
ISSN: 0724-8741
EISSN: 1573-904X 
IPA/94/1060442 
10 
10 
1500-1506 
English 
8272414 
WOS:A1993MA42100018 
IPA COPYRIGHT: ASHP The use of attenuated total reflectance Fourier transform infrared spectroscopy to obtain a quantitative concentration profile of a model permeant, 4-cyanophenol, in human stratum corneum in healthy subjects (ages 25-45 yr) is described. 4-Cyanophenol was administered for periods of 1, 2, or 3 h, either as a 10% solution in propylene glycol or in an identical vehicle that also contained 5% oleic acid. At the end of the treatment periods stratum corneum at the application site was progressively removed by adhesive tape-stripping. The presence of oleic acid in the applied formulation significantly increased the rate and extent of drug delivery as evaluated by either spectroscopy or radiochemical analysis. Furthermore, the reflectance infrared spectroscopy and direct radiochemical analysis of 4-cyanophenol as a function of stratum corneum position were highly correlated. These data strongly support the validation of the spectroscopy technique as a quantitative tool to assess percutaneous penetration in vivo. 
SKIN PENETRATION; PENETRATION ENHANCEMENT; OLEIC ACID; 4-CYANOPHENOL; INFRARED SPECTROSCOPY; TRANSDERMAL DELIVERY