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3060634 
Technical Report 
Involvement of Kupffer cell in CCl4 induced liver injury: The role of calcium 
Yang, M 
1996 
BIOSIS/96/33104 
32 
1 
75-82 
English 
BIOSIS COPYRIGHT: BIOL ABS. The hypothesis that calcium provoke O2- formation by Kupffer cells and may contribute to carbon tetrachloride (CCl4) induced liver injury was studied in SD rats. In CCl4-treated animals, hepatic malonaldehyde (nmole/gm liver) and plasma ALT (IU/ml) levels elevated significantly from 119.63|13.00 to 268.97|14.82 and from 17.3|0.18 to 806.08|37.63, respectively, compared to those in controls. Activation of Kupffer cells with high dose of retinol (250,000 IU/kg/day, po, for 7 day) significantly enhanced ALT levels, while inactivation of Kupffer cells with gadolinium chloride (7.5mg/kg/day, ip, for 2 day) attenuated the increase of serum ALT level following CCl4 treatment. Diltiazem (10 mg/kg/day, ip for 2 day) given in combination with retinol led to a marked decrease in ALT levels compare to the level in rats treated only with retinol against CCl4 treatment. In order to determine any alterations in cytochrome P450 activities, the P450 content and the CYP2E1 activity were