Supercoiled plasmid DNA as a model target for assessing the generation of free radicals at the surface of fibres | Health & Environmental Research Online (HERO)

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Citation
Tags

HERO ID

3081534

Reference Type

Journal Article

Title

Supercoiled plasmid DNA as a model target for assessing the generation of free radicals at the surface of fibres

Author(s)

Donaldson, K; Gilmour, PS; Beswick, PH

Year

1995

Is Peer Reviewed?

Yes

Journal

Experimental and Toxicologic Pathology
ISSN: 0940-2993
EISSN: 1618-1433

Publisher

GUSTAV FISCHER VERLAG JENA

Location

JENA

Report Number

BIOSIS/95/37364

Volume

Issue

Page Numbers

235-237

Language

English

PMID

8855117

DOI

10.1016/S0940-2993(11)80255-1

Web of Science Id

WOS:A1995RY05300004

Abstract

The ability of respirable amosite and crocidolite asbestos, refractory ceramic fibres (RCFs) and man made vitreous fibres (MMVFs) to cause free radical injury to plasmid, phiX174 RFI DNA was assessed. The amosite and crocidolite asbestos caused substantial damage to the DNA and, in the main, the free radicals responsible for the asbestos-mediated DNA damage were hydroxyl radicals as determined by inhibition with mannitol. Asbestos fibre-mediated damage to the DNA was completely ameliorated by the chelation of fibre-associated iron by pre-treatment of fibres with desferrioxamine-B, confirming the importance of iron in the production of free radicals. MMVFs and RCFs produced modest free radical damage to the DNA, which was prevented by mannitol but not by iron chelation.

Keywords

DNA, PLASMID; PLASMID DNA; SUPERCOILED PLASMID DNA; FREE RADICALS; RADICALS, FREE; FIBERS, SURFACE; ASBESTOS; CERAMIC FIBERS; VITREOUS FIBERS; CROCIDOLITE ASBESTOS; AMOSITE ASBESTOS; AMPHIBOLE ASBESTOS; MAN MADE FIBERS

Conference Name

5th International Inhalation Symposium

Conference Location

HANNOVER, GERMANY