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3089286 
Journal Article 
Lanthanum(III) impacts on metallothionein MTT1 and MTT2 from Tetrahymena thermophila 
Wang, Q; Xu, J; Zhu, Y; Chai, B; Liang, A; Wang, W 
2011 
Yes 
Biological Trace Element Research
ISSN: 0163-4984
EISSN: 1559-0720 
143 
3 
1808-1818 
English 
21359533 
WOS:000297348900056 
Metallothionein MTT1 and MTT2 from Tetrahymena thermophila are sulfydryl-rich proteins that can bind to and are inducible by heavy metals such as mercury, cadmium, zinc, and copper. However, little is known about the induction and binding of T. thermophila metallothionein by trivalent metals. In this study, we found that 10-80 μM La(3+) can promote Tetrahymena cells proliferation, and fluorescence spectrum analysis showed that La(3+) can enter T. thermophila cells. Real-time quantitative polymerase chain reaction showed La(3+) induced the expression of MTT1 and MTT2. Furthermore, Fluorescence analysis indicated La(3+) bind to MTT1 and MTT2. These results implied that La(3+) could interact with MTT1 and MTT2 via aspartic or glutamic acid oxygen atoms. 
Metallothionein; La(3+); MTT1; MTT2; Tetrahymena thermophila