US EPA

3096089 

Journal Article 

Peculiarities of the Clastogenic Properties of Chrysotile-Asbestos Fibers and Zeolite Particles 

Durnev, AD; Daugel-Dauge, NO; Korkina, LG; Seredenin, SB 

1993 

1 

Mutation Research
ISSN: 0027-5107
EISSN: 1873-135X 

ELSEVIER SCIENCE BV 

AMSTERDAM 

NIOSH/00218222 

319 

4 

303-308 

English 

7504204 

10.1016/0165-1218(93)90019-a 

WOS:A1993MK49400009 

https://linkinghub.elsevier.com/retrieve/pii/016512189390019A
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Peculiarities of the clastogenic properties of chrysotile (12001295) (CHR) fibers and zeolite (1318021) (ZL) particles were investigated. Peripheral blood lymphocytes (PBL) from whole blood of healthy humans were used in the in-vitro tests. Peritoneal cells and bone marrow cells from C57BL/6-mice aged 1.5 to 2 months injected intraperitoneally with 50mg/kg CHR or ZL were assessed for chromosomal aberrations (CA). Results showed that both CHR and ZL increased the level of PBLs with CA to 6.8% and 9.2%, respectively. The most frequent aberrations were chromatid breaks. The in-vivo cytogenetic tests with mice showed that CA decreased greatly on the day following injection of CHR and ZL particles, probably due to a defense reaction. By 48 hours after injection, CA reached near control levels (12% to 18%) and, by days seven and 28, CHR treated animals had CAs of 22% and 19%, respectively, while ZL treated animals developed CAs of 16.5% and 36.5%, respectively. In all cases, chromatid and chromosome breaks were predominant. The effects of antioxidant enzymes superoxide-dismutase (SOD) and catalase on the mutagenicity of CHR and ZL were investigated. Results showed that SOD reduced the frequency of CA caused by CHR, but catalase had no effect. With ZL, the inverse effects were seen. The authors conclude that reactive oxygen species take part in the mutagenic process of CHR and ZL fibers. 

Antimutagenic Agents; Asbestos, Serpentine; Mutagens; Zeolites; 1318-02-1; Catalase; EC 1.11.1.6; Superoxide Dismutase; EC 1.15.1.1; Index Medicus; Lymphocytes -- drug effects; Animals; Superoxide Dismutase -- pharmacology; Mice, Inbred C57BL; Catalase -- pharmacology; Cells, Cultured; Chromosome Aberrations; Zeolites -- antagonists & inhibitors; Zeolites -- toxicity; Asbestos, Serpentine -- antagonists & inhibitors; Mutagenicity Tests -- methods; Asbestos, Serpentine -- toxicity; Mutagens -- toxicity