Possibility of Using Rat Mesothelial Cells in Culture to Test Cytotoxicity, Clastogenicity, and Carcinogenicity of Asbestos Fibers | Health & Environmental Research Online (HERO)

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Citation
Tags

HERO ID

3097740

Reference Type

Journal Article

Title

Possibility of Using Rat Mesothelial Cells in Culture to Test Cytotoxicity, Clastogenicity, and Carcinogenicity of Asbestos Fibers

Author(s)

Jaurand, MC; Bastie-Sigeac, I; Paterour, MJ; Renier, A; Bignon, J

Year

1983

Is Peer Reviewed?

Yes

Journal

Annals of the New York Academy of Sciences
ISSN: 0077-8923
EISSN: 1749-6632

Report Number

NIOSH/00192851

Volume

407

Page Numbers

409-411

Abstract

The cytotoxicity, clastogenicity, and carcinogenicity of asbestos (1332214) fibers were examined in rat pleural mesothelial cell (PMC) cultures. PMCs from healthy rats were cultured in contact inhibited monolayers with a population doubling time (PDT) of about 30 hours and were able to metabolize benzo(a)pyrene (50328) (BaP). Cytoplasmic vacuolation and binucleated cell formation were induced by 5 to 10 micrograms per milliliter (microg/ml) of chrysotile (12001295) fibers. The PDT was increased with increasing chrysotile concentration, up to 20microg/ml, which caused a cessation of growth. Chrysotile fibers at 2microg/ml resulted in a slight but significant increase in the rate of sister chromatid exchanges. In a transformation assay using BaP as initiator, 5microg/ml of chrysotile used to treat PMCs over 11 passages of cells did not have a promoter effect. The authors conclude that rat PMCs in culture provide a useful model for the study of the interactions between asbestos and mesothelial cells.

Keywords

DCN-188271; Asbestos fibers; In vitro studies; Mesothelial cells; Cell cultures; Cytotoxic effects; Clastogens; Carcinogenesis; Dose response; Laboratory animals; 1332-21-4; 50-32-8; 12001-29-5