US EPA

3110426 

Journal Article 

Oxidative DNA Damage, Oxidative Stress and Genetic Susceptibility-Prognostic Scores in 'Missing' COPD Cases 

Gandhi, G; Kaur, G 

2015 

Yes 

International Journal of Human Genetics
ISSN: 0972-3757 

15 

3 

97-119 

WOS:000362717900002 

In situ identification of COPD cases at workplace and sample assessment for oxidative stress/DNA damage as a function of metabolic/antioxidant genotypes (as susceptibility genotypes) offers a novel manner of hazardous workplace-identification for genotoxic/carcinogenic events. In this case-control study, blood/sera samples of the COPD cases (n=32 identified spirometrically among the stone-crushing workers) and healthy controls (n=19) were assessed for 8-OHdG (DNA damage), glutathione and superoxide dismutase levels (oxidative stress) and genotyped for GSTT1, M1, P1 and MnSOD variants. Significant increase in oxidative damage and lung-function decline were observed as a function of some genotypes. Predictors of genetic damage included tGSH, SOD and GSTM1. A prognostic index score based on prognostic factors was developed revealing cases were at high (53.12%), intermediate- (34.37%) or low- (12.50%) risk for progressive DNA damage. These aberrant findings imply workplace exposure. This study provides insight on exposure-effect relationship in workers at stone-crushing sites. 

Genetic Polymorphism; Glutathione-S-Transferase; 8-OHdG; Stone-crushing Superoxide Dismutase