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HERO ID
3110634
Reference Type
Journal Article
Title
Evidence of nickel (Ni) efflux in Ni-tolerant ectomycorhizal Pisolithus albus isolated from ultramafic soil
Author(s)
Majorel, C; Hannibal, L; Ducousso, M; Lebrun, M; Jourand, P
Year
2014
Is Peer Reviewed?
Yes
Journal
Environmental Microbiology Reports
EISSN:
1758-2229
Volume
6
Issue
5
Page Numbers
510-518
DOI
10.1111/1758-2229.12176
Web of Science Id
WOS:000343798200011
Abstract
Nickel (Ni)-tolerant ectomycorrhizal Pisolithus albus was isolated from extreme ultramafic soils that are naturally rich in heavy metals. This study aimed to identify the specific molecular mechanisms associated with the response of P.albus to nickel. In presence of high concentration of nickel, P.albusNi-tolerant isolate showed a low basal accumulation of nickel in its fungal tissues and was able to perform a metal efflux mechanism. Three genes putatively involved in metal efflux were identified from the P.albus transcriptome, and their overexpression was confirmed in the mycelium that was cultivated in vitro in the presence of nickel and in fungal tissues that were sampled in situ. Cloning these genes in yeast provided significant advantages in terms of nickel tolerance (+31% Ni EC50) and growth (+83% ) compared with controls. Furthermore, nickel efflux was also detected in the transformed yeast cells. Protein sequence analysis indicated that the genes encoded a P-type-ATPase, an ABC transporter and a major facilitator superfamily permease (MFS). This study sheds light on a global mechanism of metal efflux by P.albus cells that supports nickel tolerance. These specific responses to nickel might contribute to the fungal adaptation in ultramafic soil.
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