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HERO ID
3110905
Reference Type
Journal Article
Title
Imidacloprid induces changes in the structure, genetic diversity and catabolic activity of soil microbial communities
Author(s)
Cycon, M; Markowicz, A; Borymski, S; Wojcik, M; Piotrowska-Seget, Z
Year
2013
Is Peer Reviewed?
Yes
Journal
Journal of Environmental Management
ISSN:
0301-4797
EISSN:
1095-8630
Volume
131
Page Numbers
55-65
Language
English
PMID
24140487
DOI
10.1016/j.jenvman.2013.09.041
Web of Science Id
WOS:000329422000007
Abstract
This is the first report describing the effect of imidacloprid applied at field rate (FR, 1 mg/kg of soil) and 10 times the FR (10*FR, 10 mg/kg of soil) on the structural, genetic and physiological diversity of soil bacterial community as determined by the phospholipid fatty acid (PLFA), the denaturing gradient gel electrophoresis (DGGE), and the community level physiological profile (CLPP) approaches. PLFA profiles showed that imidacloprid significantly shifted the microbial community structure and decreased the biomass of the total, bacterial and fungal PLFAs, however, this effect was transient at the FR dosage. The alterations in DGGE patterns caused by imidacloprid application, confirmed considerable changes in the overall richness and diversity of dominant bacteria. Although, as a result of imidacloprid application, the metabolic activity of microbial communities was generally lower, the richness and functional biodiversity of the soil microbial community were not negatively affected. In general, the analysis of the variance indicated that the measured parameters were significantly affected by treatment and the incubation time, however, the incubation time effect explained most of the observed variance. Imidacloprid degradation and the appearance of some new bands in DGGE profiles suggest the evolution of bacteria capable of degrading imidacloprid among indigenous microflora. (C) 2013 Elsevier Ltd. All rights reserved.
Keywords
Imidacloprid; PLFA; PCR-DGGE; CLPP; Biodegradation; Soil
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