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3118463 
Technical Report 
Separation of herbicides by high-performance liquid chromatography. Influence of water 
Gonnet, C; Rocca, JL 
1975 
PESTAB/75/2558 
Chromatogr 
2 
297-303; 1975 
French 
PESTAB. Chromatographic conditions for analysis of herbicides by high-performance liquid chromatography were examined. In all cases, a rapid preliminary analysis of the system was made using thin-layer chromatogrphy. Column passage was then performed in order to optimize the eluting power of the solvent or other parameters if necessary. The column conditions included: column, 14.5 cm x 4.6 mm; support, Lichrosorb Si 60, 5 mum; injection, 10 mul; flow rate, 6 mm/min; pressure, 65 bar; detection, UV LDC, 254 nm; mobile phase, dichloromethane saturated with water; or: column, 25 cm x 4.6 mm; support, injection and flow rate as above; pressure, 100 bar; detection, UV Zeiss 254 nm; mobile phase, dichloromethane/2-propanol (98.5:1.5). These systems, especially the system using dichloromethane modified with water, were highly selective in separating substituted thermolabile urea herbicides. Microporous supports were optimal.