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3118869 
Technical Report 
Thin-layer chromatographic separation of vernolate, suspected metabolites and derivatives 
Hermanson, HP; Siewierski, M; Winnett, AG 
1970 
HAPAB/70/02079 
Environ 
1 
38-41 
HAPAB Various thin-layer chromatographic ( TLC ) techniques for the determination and identification of vernolate ( S-propyl N,N- dipropylthiol carbamate ), its suspected metabolites and derivatives are described. The types of chromatographic solvent systems, visualizers, standard reagents, equipment and general methods employed are outlined briefly. In the TLC analysis of these compounds, the four visualizers recommended are: iodine, silver nitrate spray reagent used in Mitchell's test ( 1961 ), sodium nitroprusside utilized in the Rimini test and Congo Red spray reagent. The presence of nonvernolate components in certain technical quality herbicide preparations can be detected by using the iodine test. The developers that either failed to place vernolate away from the chromatogram extremes or did not resolve vernolate from the amines and their contaminates are mentioned. Purification of C-14-labeled vernolate was effected by using a 100:1 benzene-isopropanol solvent mixture in a thick-layer chromatographic system. When benzene was employed, the derivatives, N-phenyl N-n- propylthiourea and p-toluene-sulfonic acid, n-propylamine salt remained at the origin; when a solvent mixture of 1:1:3 water-acetic acid- acetone was used the derivatives migrated with the solvent front. ANALYSIS 70/11/00, 544 1970