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3120554 
Journal Article 
Effect of phenolic compounds from pretreated sugarcane bagasse on cellulolytic and hemicellulolytic activities 
Michelin, M; Ximenes, E; Polizeli, M; Ladisch, MR 
2016 
Yes 
Bioresource Technology
ISSN: 0960-8524
EISSN: 1873-2976 
199 
275-278 
English 
26364828 
This work shows both cellulases and hemicellulases are inhibited and deactivated by water-soluble and acetone extracted phenolics from sugarcane bagasse pretreated at 10% (w/v) for 30 min in liquid hot water at 180 or 200°C. The dissolved phenolics in vacuum filtrate increased from 1.4 to 2.4 g/L as temperature increased from 180 to 200°C. The suppression of cellulose and hemicellulose hydrolysis by phenolics is dominated by deactivation of the β-glucosidase or β-xylosidase components of cellulase and hemicellulase enzyme by acetone extract at 0.2-0.65 mg phenolics/mg enzyme protein and deactivation of cellulases and hemicellulases by the water soluble components in vacuum filtrate at 0.05-2mg/mg. Inhibition was a function of the type of enzyme and the manner in which the phenolics were extracted from the bagasse.