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3123441 
Journal Article 
Multi step FRET among three laser dyes Pyrene, Acriflavine and Rhodamine B 
Saha, J; Dey, D; Roy, AD; Bhattacharjee, D; Hussain, SA 
2016 
Yes 
Journal of Luminescence
ISSN: 0022-2313 
Elsevier 
AMSTERDAM 
172 
168-174 
English 
WOS:000370381600025 
Fluorescence Resonance Energy Transfer (FRET) system using three dyes has been demonstrated. It has been observed that multi step energy transfer occurred from Pyrene to Rhodamine B via Acriflavine. Here Acriflavine acts as an antenna to receive energy from Pyrene and transfer the same to Rhodamine B. This multi step FRET system is advantageous compared to the conventional FRET as this can be used to study molecular level interaction beyond conventional FRET distance (1-10 nm) as well as studying multi-branched macromolecules. The introduction of clay enhances the FRET efficiencies among the dye pair, which is an advantage to make the multi step system more useful. Similar approach can be used for increasing FRET efficiencies by using other dyes. © 2015 Elsevier B.V. All rights reserved. 
Multi-step FRET; Dyes; Clay