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Citation
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HERO ID
3124103
Reference Type
Journal Article
Title
Fluorescence study on the aggregation of collagen molecules in acid solution influenced by hydroxypropyl methylcellulose
Author(s)
Ding, C; Zhang, Min; Li, G
Year
2016
Is Peer Reviewed?
Yes
Journal
Carbohydrate Polymers
ISSN:
0144-8617
EISSN:
1879-1344
Publisher
ELSEVIER SCI LTD
Location
OXFORD
Volume
136
Page Numbers
224-231
Language
English
PMID
26572350
DOI
10.1016/j.carbpol.2015.09.028
Web of Science Id
WOS:000365972000029
URL
https://linkinghub.elsevier.com/retrieve/pii/S0144861715008759
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Abstract
The effect of hydroxypropyl methylcellulose (HPMC) on the aggregation of collagen molecules with collagen concentrations of 0.25, 0.5 and 1.0mg/mL was studied by fluorescence techniques. On one hand, both the synchronous fluorescence spectra and fluorescence emission spectra showed that there was no change in the fluorescence intensity of collagen intrinsic fluorescence when 30% HPMC was added, while it decreased obviously when HPMC content ≥ 50%. From the two-dimensional fluorescence correlation analysis, it was indicated that collagen molecules in 0.25 and 0.5mg/mL collagen solutions were more sensitive to HPMC than those in 1.0mg/mL collagen solution. On the other hand, the pyrene fluorescence and the fluorescence anisotropy measurements indicated that HPMC inhibited the collagen aggregation for 0.25 and 0.5mg/mL collagen, but promoted it for 1.0mg/mL collagen. The atomic force microscopy images further confirmed the effect of HPMC on collagen with different initial states.
Keywords
Collagen; Aggregation; Hydroxypropyl met hylcellulose; Fluorescence
Tags
IRIS
•
Ammonia, Oral - Problem Formulation
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