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Citation
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HERO ID
3124556
Reference Type
Journal Article
Title
Association of environmental benzo[a]pyrene exposure and DNA methylation alterations in hepatocellular carcinoma: A Chinese case-control study
Author(s)
Tian, M; Zhao, B; Zhang, Jie; Martin, FL; Huang, Q; Liu, L; Shen, H
Year
2016
Is Peer Reviewed?
1
Journal
Science of the Total Environment
ISSN:
0048-9697
EISSN:
1879-1026
Publisher
ELSEVIER
Location
AMSTERDAM
Volume
541
Page Numbers
1243-1252
Language
English
PMID
26476064
DOI
10.1016/j.scitotenv.2015.10.003
Web of Science Id
WOS:000365289300127
URL
https://linkinghub.elsevier.com/retrieve/pii/S0048969715308202
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Abstract
Epidemiological studies implicate environmental risk factors and epigenetic alterations in the multistage process of hepatocellular carcinoma (HCC) development. However, associations between environmental factors and DNA methylation of tumour suppressor genes (TSGs) in HCC development remain ambiguous. Understanding how possible interactions influence risk may provide insights into the complexity of hepato-carcinogenesis. For this study, blood samples were collected from HCC patients (n=90) and healthy volunteers (n=99) from Xiamen (China) and data for selected environmental risk factors [e.g., benzo[a]pyrene (B[a]P), hepatitis B or C virus (HBV or HCV) infection, smoking and alcohol consumption] were recorded; factors identified as significantly higher (P<0.05) amongst case subjects compared to controls were identified. In order to assess associations for epigenetic alterations and HCC risk factors, serum DNA methylation of TSGs was quantified using high-resolution melting (HRM) analysis. Our results clearly indicate elevated methylation patterns for detoxification gene [glutathione-S-transferase Pi (GSTP)] promoter regions in cases compared to control subjects. Additionally, GSTP promoter hypermethylation and B[a]P diol epoxide-albumin (BPDE-Alb) were positively correlated with HCC incidence. Our epidemiological and in vitro cell model studies indicated that GSTP promoter DNA methylation regulates this gene's expression. Moreover, GSTP also plays an important role in B[a]P detoxification and potential protective role against B[a]P-induced liver cell toxicity and hepato-carcinogenesis.
Keywords
B[a]P; Case control study; DNA methylation; Epigenetic; GSTP; Hepatocellular carcinoma
Tags
IRIS
•
Ammonia, Oral - Problem Formulation
•
Benzo(a)pyrene (BaP)
August 2016 Update
Human Studies
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