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3159054 
Journal Article 
A highly sensitive aptasensor for OTA detection based on hybridization chain reaction and fluorescent perylene probe 
Wang, B; Wu, Y; Chen, Y; Weng, B; Xu, L; Li, C 
2016 
Yes 
Biosensors and Bioelectronics
ISSN: 0956-5663
EISSN: 1873-4235 
81 
125-130 
English 
26938491 
WOS:000374811800019 
is supplemented by 5029474 [Supplement]
An optical aptasensor was developed for ultrasensitive detection of ochratoxin A (OTA) based on hybridization chain reaction (HCR) amplification strategy and fluorescent perylene probe (PAPDI)/DNA composites. Dendritic DNA concatamers were synthesized by HCR strategy and modified on magnetic nanoparticles through aptamer as medium. A large amount of PAPDI probe aggregated under the induction of DNA concatamers and caused fluorescence quenching. In the presence of OTA, the PAPDI/DNA composites were released from magnetic nanoparticles due to the strong affinity between aptamer and OTA. In ethanol, PAPDI monomers disaggregated and produced strong fluorescence. The present method displays excellent sensitivity and selectivity towards OTA.