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3208864 
Journal Article 
Waste cooking oil: A new substrate for carotene production by Blakeslea trispora in submerged fermentation 
Nanou, K; Roukas, T 
2016 
Yes 
Bioresource Technology
ISSN: 0960-8524
EISSN: 1873-2976 
203 
198-203 
English 
26724551 
The objective of this study was to evaluate a waste, waste cooking oil (WCO) as substrate for carotene production by Blakeslea trispora in shake flask culture. WCO was found to be a useful substrate for carotene production. B. trispora formed only pellets during fermentation. The oxidative stress in B. trispora induced by hydroperoxides and BHT as evidenced by increase of the specific activities of superoxide dismutase (SOD) and catalase (CAT) increased significantly the production of carotenes. The highest concentration of carotenes (2021±75mg/l or 49.3±0.2mg/g dry biomass) was obtained in culture grown in WCO (50.0g/l) supplemented with CSL (80.0g/l) and BHT (4.0g/l). In this case the carotenes produced consisted of β-carotene (74.2%), γ-carotene (23.2%), and lycopene (2.6%). The external addition in the above medium glucose, Span 80, yeast extract, casein acid hydrolysate, l-asparagine, thiamine. HCl, KH2PO4, and MgSO4·7H2O did not improve the production of carotenes.