US EPA

3232157 

Journal Article 

Antioxidant activity of hydro-alcoholic extracts of 4 citrus species flower 

Nabavi, SF; Nabavi, SM; Ebrahimzadeh, MAli 

2016 

1 

Progress in Nutrition
ISSN: 1129-8723 

18 

1 

74-80 

WOS:000373213000011 

Objectives: During past two decades, much attention has
been paid to antioxidant activity of different edible parts of medicinal plants. Present study
aimed to evaluate antioxidant activity of hydro-alcoholic extract of flowers of Citrus aurantium,
Citrus nobilis, Citrus limon and Citrus sinensis. Methods: Biological activities of hydro-
alcoholic extract of Citrus aurantium, Citrus nobilis, Citrus limon and Citrus sinensis flowers
were investigated employing different in vivo and in vitro assay systems including DPPH, nitric
oxide and H2O2 scavenging models, Fe2+ chelating, reducing power model, linoleic acid system
test, as well as H2O2-induced hemolysis test. Results: Citrus limon extract showed best activity
in DPPH and nitric oxide radicals scavenging activity and Fe2+ chelating activity than others.
Citrus nobilis extract exhibited better H2O2 scavenging. Extracts exhibited good antioxidant
activity in linoleic acid system test that were comparable with vitamin C (p>0.05). The extracts
showed weak reducing power activity. Citrus sinensis showed better antihemolytic activity against
H2O2 induced hemolysis. Conclusion: Combining data showed that hydro-alcoholic extract of flowers
of 4 Citrus species possess antioxidant activity in different assay systems. 

antioxidant; citrus; flower extract; free radical scavenging; lipid peroxidation