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3237153 
Journal Article 
Prolongation of alloskin graft survival by catalytic scavengers of reactive oxygen species 
Tocco, G; Illigens, B; Malfroy, B; Benichou, G 
2006 
Yes 
Cellular Immunology
ISSN: 0008-8749
EISSN: 1090-2163 
241 
2 
59-65 
English 
16970930 
WOS:000242003100001 
We tested the effects of salen manganese (Salen-Mn)
complexes, which are scavengers of reactive oxygen species exhibiting superoxide dismutase and
catalase activities on the rejection of and alloresponse to fully allogeneic skin grafts in mice.
We showed that pre-transplant treatment of C57B1/6 don Dr skin or of BALB/c recipients with
Salen-Mn complexes significantly delayed allograft rejection. ELISPOT analysis of alloimmune
response of treated mice revealed a significant reduction of the frequency of type 1 cytokine
(proinflammatory) producing T-cells, while the number of activated T-cells producing type 2
cytokines was elevated. In addition, anti-oxidative treatment of graft recipients resulted in a
profound inhibition of their donor-specific cytotoxic T-cell response. Our results indicate that
salen manganese complexes mediate their effect on graft rejection both by reducing the
susceptibility of graft tissue to ROS-mediated injury and by exerting an anti-inflammatory effect
in recipients. (c) 2006 Published by Elsevier Inc. 
allotransplantation; cytokines; reactive oxygen species; skin graft; T-cells