Health & Environmental Research Online (HERO)


Print Feedback Export to File
3238614 
Journal Article 
Role of combined L-arginine and prostaglandin E-1 in renal ischemia-reperfusion injury 
Mahmoud, IM; Hussein, A; Sarhan, ME; Awad, A; El Desoky, I 
2007 
1 
Nephron Physiology
ISSN: 1660-2137 
105 
4 
57-65 
English 
17337910 
WOS:000246393400001 
Background: L-Arginine (L-arg) and Prostaglandin E-1 (PGE
(1)) have been used effectively as single agents to ameliorate renal ischemia-reperfusion injury.
We hypothesized that combined treatment with L-arg and PGE(1) would be more effective. Materials
and Methods: The left renal artery of male Sprague-Dawley rats was clamped for 45 min and the
right kidney was removed. Fifty six rats were randomly allocated into 5 groups each consisted of
12 rats except sham group (n = 8). (1) sham, underwent right nephrectomy only; (2) control,
untreated ischemic rats; (3) L-arg group, L-arg-treated ischemic rats; (4) PGE(1) group, PGE(1)-
treated ischemic rats; (5) L-arg+PGE(1) group, ischemic rats treated with both L-arg and PGE(1).
Renal function and histology were assessed on days 2 and 7 postoperatively. Results: All rats,
except control ones, showed a significant improvement of renal function towards normal on
postoperative day 7. Serum creatinine and creatinine clearance were significantly better in L-
arg+PGE(1) group compared to all other groups on day 7. With the exception of sham-operated and
L-arg+PGE(1)-treated animals, all other groups showed significant increases in fractional
excretion of sodium (FENa) in response to renal ischemia-reperfusion. The severest tubular damage
was determined in the kidneys of control rats. Rats treated with L-arg+PGE(1) had the least
severe tubular damage. Conclusion: The administration of either L-arg or PGE(1) attenuates both
functional and structural consequences of renal warm ischemia. A near total protection might be
achieved when both agents are administered concomitantly.