Enzymological aspects of bioconversion of m-dinitrobenzene | Health & Environmental Research Online (HERO)

Health & Environmental Research Online (HERO)

Print Feedback Export to File

This record has one attached file:

Citation
Tags

HERO ID

3239345

Reference Type

Journal Article

Title

Enzymological aspects of bioconversion of m-dinitrobenzene

Author(s)

Dey, S

Year

2002

Is Peer Reviewed?

Yes

Journal

Journal of Environmental Biology
ISSN: 0254-8704
EISSN: 2394-0379

Volume

Issue

Page Numbers

289-294

Language

English

PMID

12597574

Web of Science Id

WOS:000177407700013

Abstract

Three main enzymes, responsible for bioconversion of 1,3
dinitrobenzene (m-DNB) by Micrococcus colpogenes MCM B 410, were isolated from the sonicated cell
mass, grown in presence of m-DNB in a synthetic medium, for 7 days. The soluble proteins were
separated by differential precipitation and also separated by native PAGE. Each fraction obtained
from both the protocols, was tested for nitro aryl reductase, aryl mono oxygenase and resorcinol
2,3 dioxygenase. The apparent molecular weight of the proteins were nitro aryl reductase (3D
kDa), aryl mono oxygenase (110 kDa) and resorcinol 2,3 di oxygenase (65 kDa).

Keywords

enzymes; biodegradation; m-DNB; micrococcus colpogenes