US EPA

3239731 

Journal Article 

Protective effect of persimmon peel polyphenol against high glucose-induced oxidative stress in LLC-PK1 cells 

Yokozawa, T; Kim, YAe; Kim, HY; Lee, YA; Nonaka, GenI 

2007 

Yes 

Food and Chemical Toxicology
ISSN: 0278-6915
EISSN: 1873-6351 

45 

10 

1979-1987 

10.1016/j.fct.2007.04.018 

WOS:000249598500024 

The effect of persimmon peel polyphenol (PPP) on high
glucose-induced oxidative stress was investigated using LLG-PK1 cells, which is susceptible to
oxidative stress. High-concentration glucose (30 mM) treatment induced LLC-PK1 cell death, but
high molecular-PPP (HMPPP) and low molecular-PPP (LMPPP), at concentrations of 5 or 10 mu g/ml,
significantly inhibited the high glucose-induced cytotoxicity. Furthermore, treatment with HMPPP
or LMPPP dose-dependently reduced the intracellular reactive oxygen species level increased by 30
mM glucose. In addition, nitric oxide, superoxide and peroxynitrite levels were increased by 30
mM glucose treatment, but they were concentration-dependently inhibited by HMPPP or LMPPP
treatment. High glucose levels induced the overexpressions of inducible nitric oxide synthase
(NOS) and cyclooxygenase-2 (COX-2) proteins, but HMPPP or LMPPP treatment reduced the
overexpressions of these proteins. HMPPP or LMPPP also inhibited the nuclear translocation of
nuclear factor-kappa B (NF-kappa B) induced by 30 mM glucose in LLG-PK1 cells. In particular,
LMPPP exhibited stronger inhibitory activities on high glucose induced oxidative stress than
HMPPP. These findings indicate the potential benefits of persimmon peel as a valuable source of
antioxidants in the diabetic condition which will reduce the oxidative stress induced by
hyperglycemia. (c) 2007 Elsevier Ltd. All rights reserved. 

persimmon peel; high glucose; oxidative stress; reactive oxygen species; iNOS; COX-2; NF-kappa B