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3450817 
Journal Article 
High contaminant loads in Lake Apopka's riparian wetland disrupt gene networks involved in reproduction and immune function in largemouth bass 
Martyniuk, CJ; Doperalski, NJ; Prucha, MS; Zhang, JL; Kroll, KJ; Conrow, R; Barber, DS; Denslow, ND 
2016 
Yes 
Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics
ISSN: 1744-117X
EISSN: 1878-0407 
Elsevier Inc. 
NEW YORK 
19 
140-150 
English 
27397556 
WOS:000382416300016 
Lake Apopka (FL, USA) has elevated levels of some organochlorine pesticides in its sediments and a portion of its watershed has been designated a US Environmental Protection Agency Superfund site. This study assessed reproductive endpoints in Florida largemouth bass (LMB) (Micropterus salmoides floridanus) after placement into experimental ponds adjacent to Lake Apopka. LMB collected from a clean reference site (DeLeon Springs) were stocked at two periods of time into ponds constructed in former farm fields on the north shore of the lake. LMB were stocked during early and late oogenesis to determine if there were different effects of contamination on LMB that may be attributed to their reproductive stage. LMB inhabiting the ponds for ~4months had anywhere from 2 to 800 times higher contaminant load for a number of organochlorine pesticides (e.g. p, p'-DDE, methoxychlor) compared to control animals. Gonadosomatic index and plasma vitellogenin were not different between reproductively-stage matched LMB collected at reference sites compared to those inhabiting the ponds. However, plasma 17β-estradiol was lower in LMB inhabiting the Apopka ponds compared to ovary stage-matched LMB from the St. Johns River, a site used as a reference site. Sub-network enrichment analysis revealed that genes related to reproduction (granulosa function, oocyte development), endocrine function (steroid metabolism, hormone biosynthesis), and immune function (T cell suppression, leukocyte accumulation) were differentially expressed in the ovaries of LMB placed into the ponds. These data suggest that (1) LMB inhabiting the Apopka ponds showed disrupted reproduction and immune responses and that (2) gene expression profiles provided site-specific information by discriminating LMB from different macro-habitats. 
Environmental genomics; Environmental monitoring; Field study; Organochlorine pesticides; Sub-network enrichment analysis; 1,1 dichloro 2,2 bis(4 chlorophenyl)ethylene; hormone; methoxychlor; organochlorine pesticide; steroid; vitellogenin; transcriptome; vitellogenin; water pollutant; animal experiment; animal tissue; Article; controlled study; developmental stage; endocrine function; environmental impact; female; Florida; gene disruption; gene expression; gene expression profiling; gene regulatory network; gonad; hormone synthesis; immune response; immune system; lake; largemouth bass; leukocyte; nonhuman; ovary; plasma; pond; priority journal; reproduction; riparian ecosystem; species habitat; steroid metabolism; T lymphocyte; transcriptomics; water contamination; wetland; animal; bass; biology; blood; cellular immunity; drug effects; gene regulatory network; genetics; growth, development and aging; microarray analysis; pharmacology; reproduction; water pollutant; wetland; Animals; Bass; Computational Biology; Gene Regulatory Networks; Immunity, Cellular; Lakes; Microarray Analysis; Reproduction; Transcriptome; Vitellogenins; Water Pollutants, Chemical; Wetlands