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3514968 
Journal Article 
Inhibitory effect of a Brazilian marine brown alga Spatoglossum schroederi on biological activities of Lachesis muta snake venom 
Souza Domingos, TF; Ortiz-Ramirez, FA; Villaca, RC; Cavalcanti, DN; Sanchez, EF; Teixeira, VL; Fuly, AL 
2012 
0 
Revista Brasileira de Farmacognosia
ISSN: 0102-695X 
Sociedade Brasileira de Farmacognosia 
22 
4 
741-747 
English 
WOS:000305981800006 
The ability of crude extracts of the brown seaweed
Spatoglossum schroederi to counteract some of the biological activities of Lachesis muta snake
venom was evaluated. In vitro assays showed that only the extract of S. schroederi prepared in
ethyl acetate was able to inhibit the clotting of fibrinogen induced by L. muta venom. On the
other hand, all extracts were able to inhibit partially the hemolysis caused by venom and those
prepared in dichloromethane or ethyl acetate fully neutralized the proteolysis and hemorrhage
produced by the venom. Moreover, the dichloromethane or ethyl acetate extracts inhibited the
hemolysis induced by an isolated phospholipase A(2) from L. muta venom, called LM-PLA(2)-I. In
contrast, the hexane extract failed to protect mice from hemorrhage or to inhibit proteolysis and
clotting. These results show that the polarity of the solvent used to prepare the extracts of S.
schroederi algae influenced the potency of the inhibitory effect of the biological activities
induced by L. muta venom. Thus, the seaweed S. schroederi may be a promising source of natural
inhibitors of the enzymes involved in biological activities of L. muta venom. 
Spatoglossum schroederi; seaweed; Lachesis muta; snake venom; bioprospecting