US EPA

3531121 

Journal Article 

Decreased asbestos-induced lung inflammation and fibrosis after radiation and bone marrow transplant 

Levis, J; Loi, R; Butnor, KJ; Vacek, P; Steele, C; Mossman, BT; Weiss, DJ 

2008 

Yes 

American Journal of Respiratory Cell and Molecular Biology
ISSN: 1044-1549
EISSN: 1535-4989 

AMER THORACIC SOC 

NEW YORK 

38 

1 

16-25 

English 

17673685 

10.1165/rcmb.2007-0249OC 

WOS:000251845200003 

The effect of lung irradiation on subsequent inflammatory or fibrotic lung injuries remains poorly understood. We postulated that irradiation and bone marrow transplantation might impact the development and progression of lung remodeling resulting from asbestos inhalation. Our objective was to determine whether irradiation and bone marrow transplantation affected inflammation and fibrosis associated with inhaled asbestos exposure. Inflammation, cytokine production, and fibrosis were assessed in lungs of naïve and sex-mismatched chimeric mice exposed to asbestos for 3, 9, or 40 days. Potential engraftment of donor-derived cells in recipient lungs was examined by fluorescence in situ hybridization and immunohistochemistry. Compared with asbestos-exposed naïve (nonchimeric) mice, chimeric mice exposed to asbestos for 3, 9, or 40 days demonstrated significant abrogation of acute increases in asbestos-associated inflammatory mediators and fibrosis. Donor-derived cells trafficked to lung but did not significantly engraft as phenotypic lung cells. Irradiation and bone marrow transplantation alters inflammatory and fibrotic responses to asbestos, likely through modulation of soluble inflammatory mediators. 

Cytokines; Inflammation Mediators; Asbestos; 1332-21-4; Index Medicus; Lung -- metabolism; Inflammation -- pathology; Lung -- pathology; Cell Movement -- drug effects; Transplantation, Homologous; Animals; Inflammation -- metabolism; Time Factors; Inflammation -- etiology; Cell Movement -- radiation effects; Whole-Body Irradiation; Asbestosis -- pathology; Cytokines -- biosynthesis; Pneumonia -- pathology; Transplantation Chimera -- metabolism; Pneumonia -- metabolism; Asbestosis -- metabolism; Pulmonary Fibrosis -- metabolism; Bone Marrow Transplantation; Pneumonia -- etiology; Gamma Rays -- adverse effects; Pulmonary Fibrosis -- etiology; Pulmonary Fibrosis -- pathology; Inflammation Mediators -- metabolism; Asbestos -- toxicity