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359728 
Journal Article 
Gill damage, metallothionein gene expression and metal accumulation in Tilapia sparrmanii from selected field sites at Rustenburg and Potchefstroom, South Africa 
Heerden Dv; Rensburg PJv; Nikinmaa, M; Vosloo, A 
2006 
31 
89-98 
Fish were collected from field sites in the mining and agricultural areas of Potchefstroom and Rustenburg, North-West Province, South Africa. Water and sediment samples from each site, together with fish muscle and gills, were analysed by Inductively Coupled Plasma Mass Spectrometry (ICP-MS) for Li, Na, Mg, Al, Ca, Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Cd, Hg, Pb and U. All metal levels were within the levels set by the South African Department of Water Affairs and Forestry, except for copper, which was still within the levels set by other guidelines. Water hardness, pH, dissolved oxygen concentration and conductivity were measured at each site. Fish gills were analysed morphometrically for arithmetic mean epithelial thickness (Har) and with Reverse Transcriptase PCR for metallothionein (MT) gene expression levels. Fish liver was also analysed for MT expression. Har was found to increase with changing copper levels in gills of fish, while gill and liver MT levels corresponded to changes in sediment and gill cadmium levels, respectively. There were highly significant differences in Har between different sampling sites, while no significant differences in gill and liver MT expression were found. 
aluminium; arsenic; calcium; chromium; cobalt; copper; gene expression; genes; gills; iron; lead; lithium; magnesium; manganese; mass spectrometry; metallothionein; nickel; pH; sodium; uranium; water hardness; water quality; zinc; South Africa; fishes; Tilapia sparrmanii; vertebrates; Chordata; animals; aquatic organisms; aquatic animals; eukaryotes; Tilapia; Cichlidae; Perciformes; Osteichthyes; Southern Africa; Africa South of Sahara; Africa; Developing Countries; Threshold Countries; Anglophone Africa; Commonwealth of Nations; aluminum; hydrogen ion concentration; Mn; potential of hydrogen; water composition and quality; Animal Genetics and Breeding (LL240) (New March 2000); Toxicology and Poisoning of Animals (LL950) (New March 2000); Aquatic Biology and Ecology (MM300); Water Resources (PP200)