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3658454 
Journal Article 
Antioncogenic Activity of Diverse Chemical Carcinogens on Retrovirus-Induced Transformation of Human Skin Fibroblasts 
Blakeslee, JR; Elliot, AM; Carter, LJ 
1983 
Yes 
Annals of the New York Academy of Sciences
ISSN: 0077-8923
EISSN: 1749-6632 
NIOSH/00192598 
Annals of the New York Academy of Sciences 
407 
1 
412-415 
English 
Several classes of carcinogenic and noncarcinogenic compounds were studied to determine their effects on viral gene function. Detroit-550 normal male human skin fibroblasts (HSFs) were cultured, and ST-Fe-SV virus was titrated in HSFs. HSF preconfluent log phase growth cells were infected with 20 focus forming units per well of 40,000 cells, before or after treatment with nontoxic doses of compounds of known carcinogenic activity in rodents. Chemical exposures lasted 30 minutes, after which they were washed and refed with medium. Foci were enumerated in fixed cells at 7 to 10 days after infection. Cells were most affected between 2 and 6 hours after infection. Virus transformation was demonstrated in cells treated with carcinogenic naphthyl amines, polycyclic aromatic hydrocarbons, hydrazines, and other carcinogenic and noncarcinogenic compounds. No virus transformation was noted in cells treated with noncarcinogenic compounds. The authors conclude that these data correlate with the carcinogenicity data for the test compounds. 
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