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3722392 
Journal Article 
ENZYME ACTIVITY IN SOYBEAN SEEDS PRODUCED UNDER FOLIAR APPLICATION OF MANGANESE 
Carvalho, ER; Oliveira, JA; de Resende Von Pinho, EV; Neto, JC 
2014 
0 
Ciencia e Agrotecnologia
ISSN: 1413-7054 
38 
4 
317-327 
WOS:000342066700001 
Several factors affect the quality of soybean seeds, including the mineral nutrition of plants. Manganese (Mn) is an important nutrient because it has as one of its functions the enzyme activation. The aim of this study was to evaluate enzyme activity in seeds of soybean cultivars produced with foliar application of different doses of Mn. The experiment was conducted at the Universidade Federal de Lavras (UFLA) (Federal University of Lavras) in randomized blocks with three replications and 4 x 4 x 2 factorial arrangement consisting of four soybean cultivars, two conventional and its genetically modified RR derived (BRS Celeste and BRS Baliza RR; BRSGO Jatai and BRS Silvania RR), four doses of Mn via foliar application (0; 200; 400 and 600 g Mn ha(-1)) and two stages of application (R-1 or R-3). In the seeds, the expressions of the enzymes esterase (EST), malate dehydrogenase (MDH), alcohol dehydrogenase (ADH), superoxide dismutase (SOD), peroxidase (PRX) and isocitratelyase (ICL) were determined. For evaluation of physiological quality, the germination test and emergence speed index (ESI) were performed. The Mn content in the seeds was also determined. The expression of the enzymes EST, SOD, PRX and ICL in soybean seeds are affected by foliar application of Mn, regardless of the stages of application. In the seeds of the cultivars that showed better physiological quality, Celeste and Baliza RR, greater expressions of the enzymes ADH and ICL and lower expressions of MDH and of PRX were observed. 
Enzymes; Glycine max; quality; micronutrients