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3814093 
Journal Article 
[Determination of roxithromycin in serum by high-performance liquid chromatography with ultraviolet detection] 
Qin, Y; Zou, Y; Liang, M; Huang, Y; Yu, Q; Feng, P 
2001 
0 
Huaxi Yike Daxue Xuebao / Journal of West China University of Medical Sciences
ISSN: 0257-7712 
32 
4 
612-614 
Chinese 
12528565 
OBJECTIVE: To make better the RP-HPLC method for the determination of roxithromycin(RM) in human serum.

METHODS: RM and clarithromycin (internal standard) were extracted from alkalinized serum sample (500 microliters) with methylene chloride. After evaporation of the organic layer, the residue was dissolved in 100 microliters of acetonitrile-ammonium phosphate (1:1, pH 6.0) and washed with n-hexane, then 20 microliters was injected onto a column (5 microns, 15 cm x 4.6 mm) of Penomenex luna C18. The mobile phase was acetonitrile-0.05 mol/L phosphoric acid (39:19:42, adjusted to pH 7.2 with ammonia water) pumped at 1.2 ml/min through the column. The variable wavelength UV detector operated at 0.01 aufs and the wavelength was set at 210 nm.

RESULTS: The retention times for RM and clarithromycin were 4.4 min and 5.0 min, respectively. Standard curve was linear in the concentration range of 0.25 to 32 mg/L. The detection limit in serum was 0.06 mg/L; the average method recovery 97.4%; the inter-day RSD less than 3.0%.

CONCLUSION: This method was found to be simple, rapid, sensitive and accurate for determination of RM in human serum. 
Roxithromycin; RP-HPLC; Serum drug concentration