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3983780 
Journal Article 
Effect of 3,5,3 '-triiodo-L-thyronine on amino acid accumulation and membrane potential in Sertoli cells of the rat testis 
Silva, F; Leite, LD; Barreto, KP; D'Agostini, C; Zamoner, A 
2001 
1 
Life Sciences
ISSN: 0024-3205
EISSN: 1879-0631 
69 
8 
977-986 
English 
11488410 
WOS:000169913800011 
The purpose of this study was to investigate the effect of T-3 on amino acid accumulation and on the membrane potential of Sertoli cells of immature rat testes. Testes of pre-pubertal and pubertal rats were pre-incubated (30 min) in Krebs-Ringer bicarbonate buffer and incubated in the presence of [C-14]methylaminoisobutyric acid with and without T-3 for 15, 45 and 60 min. The hormone (10(-6) M and 10(-7)M) significantly stimulated amino acid accumulation in 6 and 13-day old rat testes but did not have any effect in neonatal and pubertal animals, T-3 produced a dose-dependent hyperpolarizing effect at concentrations of 10(-6) M, 10(-5) M, 2 X 10(-5) M and 10(-4) M. We conclude that T-3 induces a membrane hyperpolarization in Sertoli cells and stimulates amino acid accumulation in immature rat testes, demonstrating that the hormone has a rapid plasma membrane action, (C) 2001 Elsevier Science Inc. All rights reserved. 
T-3; amino acid accumulation; membrane potential; Sertoli cell; thyroid-hormone; chloride currents; protein-synthesis; ca-45(2+) uptake; calcium; transport; stimulation; retinol; lactotrophs; messenger; Research & Experimental Medicine; Pharmacology & Pharmacy; 0 (Amino Acids); 06LU7C9H1V (Triiodothyronine); 11P2JDE17B (beta-Alanine); 19036-43-2 (2,2-dimethyl-beta-alanine); SY7Q814VUP (Calcium); Amino Acids/ metabolism; Animals; Calcium/metabolism; Membrane Potentials/ drug effects; Rats, Wistar; Sertoli Cells/drug effects/metabolism; Sexual Maturation; Testis/ drug effects; Triiodothyronine/ pharmacology; beta-Alanine/ analogs & derivatives/metabolism