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3997548 
Journal Article 
Collagen intermingled chitosan-tripolyphosphate nano/micro fibrous scaffolds for tissue-engineering application 
Pati, F; Adhikari, B; Dhara, S 
2012 
Yes 
Journal of Biomaterials Science, Polymer Edition
ISSN: 0920-5063
EISSN: 1568-5624 
Taylor & Francis Group Ltd., 2 Park Square Oxford OX14 4RN United Kingdom 
23 
15 
1923-1938 
English 
21967759 
WOS:000308237700004 
Scaffolds comprising a nano- and micro-fibrous architecture are promising for tissue engineering, where nanofibers act as connecting network among microfibers and provide a 3D structural environment and mechanical stability for facilitating cell attachment, proliferation and migration. In this study, a novel structure was developed with polymeric micro and nano combined fibrous architecture, which aims to mimic the native extracellular matrix for tissue regeneration. Chitosan-tripolyphosphate (TPP) microfibers were prepared by wet spinning method, where collagen solution was allowed to self-assemble into nano/microfibers and subsequently freeze-dried for obtaining this combined architecture. To ensure prolonged mechanical stability, the scaffold was cross-linked using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS). This polymeric nano/micro combined scaffold revealed remarkable cellular activity and cytocompatibility towards both fibroblasts and osteoblast like cells and supported improved attachment and proliferation of cells than that of bare chitosan-TPP scaffolds owing to the presence of a bioactive molecule, collagen, in the intermingled form with chitosan-TPP microfiber. 
Biotechnology and Bioengineering Abstracts; Collagen; chitosan; intermingled scaffold; cell attachment; cell migration; Cell proliferation; scaffolds; Tissue engineering; Cell adhesion; Fibroblasts; Extracellular matrix; carbodiimide; Spinning; Osteoblasts; Cell migration; W 30920:Tissue Engineering