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4001730 
Journal Article 
Validated HPLC-UV method for the determination of berberine in raw herb Daruharidra (Berberis aristata DC), its extract, and in commercially marketed Ayurvedic dosage forms 
Pasrija, A; Singh, R; Katiyar, CK 
2010 
Yes 
International Journal of Ayurveda Research
ISSN: 0974-7788 
1 
4 
243-246 
English 
21455453 
A new, simple, sensitive, selective, and precise high-performance liquid chromatography (HPLC) method for analysis of berberine in crude plant material, herbal extract, and ayurvedic dosage forms was developed and validated. The stationary phase was inert sil C(18) column The mobile phase consisting of acetonitrile (HPLC Grade) and potassium dihydrogen phosphate buffer (pH 2.5) in a gradient flow was used.The column was equilibrated with the mobile phase (flow rate 1.0 ml/min); elution was monitored at 346 nm. The linear regression analysis data for the calibration plots showed good linear relationship, with r(2)= 0.9942 in the concentration range of 16380-30420 μg/ml with respect to the peak area. The method was validated for specificity, precision, recovery, and linearity according to the International Conference on Harmonization guidelines. Statistical analysis of the data showed that the method is reproducible and selective for the estimation of berberine.