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4091385 
Technical Report 
Pulsed Electric Fields for Biological Weapons Defense 
Gundersen, MA 
2008 
NTIS/03430034 
GRA and I 
GRA and I 
Pulsed power for biological investigations newly developed at USC include a fast diode-based systems designed to drive cell suspensions in a microscope slide electrode microchamber for observations of living cells during pulse exposure with pulse durations from 3 ns to 30 ns and electric fields from 1 MV/m to 10 MV/m. Nanoelectropulse responses have been observed in vitro with the following cell lines (human unless otherwise noted; ATCC catalog number in parentheses): Jurkat T lymphoblasts (TIB-152), RPMI 8226 multiple myeloma cells (CCL-155), SKOV-3 ovarian cancer (HTB-77), AsPci pancreatic cancer cells (CRL- 1682), U-S7 MG glioblastoma cells (HTB-14), MCF-7 breast adenocarcinoma (HTB- 22), WI-38 fetal lung fibroblasts (CCL-75), WI-38 VA-13 sub-line 2RA (SV4O- transformed WI-38; CCL-75.1), C6 rat glioma cells (CCL-107), NIH 3T3 murine fibroblasts (CRL-1658), normal T cells (from healthy donors), and bovine adrenal chromaffin cells and rabbit cardiomyocytes (both primary cultures). AsPcl pancreatic cancer cells were also implanted into athymic nude mice for evaluation of solid tumor responses in vivo.