Isola, DA; Rogers, RE; Ansari, R; Smith, LW
This study was designed to assess human respiratory exposure to a surrogate fine fragrance and address concerns about asthma, lung disease, and neurological effects. The test fragrance reflected a product with a low level of scent over time after topical application by an atomizer. The formulation contained 2.2% each of benzyl acetate (BA), eugenol (EU), alpha-hexylcinnamaldehyde (HCA), 1, 3, 4, 6, 7, 8-hexahydro- 4, 6, 6, 7, 8, 8-hexamethylcyclopenta-gamma-benzopyran (HHCB), hydroxycitronellal (HO-C), ?-ionone (?-I), d-limonene (d-L), linalool (LL), and methyl dihydrojasmonate (MDJ) and 80% of a vehicle (80% EtOH and 20% HOH). The fragrance materials were chosen based on volatility, chemical structure, toxicity, and volume of use. The test product was sprayed toward a manikin at a distance of 3.5 inches. Three different anatomical areas were sprayed with 3 pump actuations each. The concentration of each fragrance material was measured at the adult-breathing zone (5 ft) and the child-breathing zone (1.5 ft) from the start of spray until 5 hrs post-spray. Particle size analysis was also conducted. The samples were collected on thermal desorption tubes and analyzed by GC/MS. The data showed that 0.89 g of test material was released after 9 actuations. Peak total fragrance air concentrations of 1256 mg/m3 (adult zone) and 850 mg/m3 (child zone) were seen at 8-18 min post spray. The more volatile fragrance materials (d-L, LL, BA) peaked at that time. The less volatile fragrance materials (beta-I, MDJ, HCA, HHCB, EU, HO-C) peaked at later time periods, generally. Previous studies with air fresheners showed peak fragrance air concentrations of 2550 mg/m3 (adult zone)/2070 mg/m3 (child zone) at 1 min for aerosols and 2286 mg/m3 at 1 hr for plug-ins. The product type and volatility of each fragrance material affect air concentration. The exposure data will be used for clinical study design.
