Health & Environmental Research Online (HERO)


Print Feedback Export to File
4095017 
Journal Article 
Acrolein detection based on alcohol dehydrogenase inhibition 
Covaci, OI; Bucur, B; Radu, GL 
2013 
Yes 
International Journal of Environmental Analytical Chemistry
ISSN: 0306-7319
EISSN: 1029-0397 
93 
3 
325-334 
WOS:000313671100006 
This paper presents the effect of acrolein on three dehydrogenases and proposes a fast spectrometric method for acrolein analysis. We have found that alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (AlDH) are inhibited by low acrolein concentrations (0.2?mM) while inhibition of glutamate dehydrogenase (GDH) is not observed even at higher acrolein concentrations (1?mM). Acrolein is a suicide substrate for AlDH and ADH inhibition by acrolein is competitive. Cysteine (L-Cys) and glutathione (GSH) react with acrolein and thus reduce its expected inhibitory effect. ADH was chosen to develop a spectrophotometric method for acrolein analysis based on enzyme inhibition. The calibration curve is linear between 0.2 and 1.0?mM acrolein. 
acrolein inhibition; acrolein analysis; alcohol dehydrogenase; aldehyde dehydrogenase; glutamate dehydrogenase