Health & Environmental Research Online (HERO)


Print Feedback Export to File
4106100 
Journal Article 
Survivin mRNA-circulating tumor cells are associated with prostate cancer metastasis 
Wang, H; Yang, M; Xu, J; Zou, B; Zhou, Q; Bian, J; Wang, X 
2016 
1 
Tumor Biology
ISSN: 1010-4283
EISSN: 1423-0380 
37 
1 
723-727 
English 
26242261 
WOS:000374576200078 
has retraction 4098513 Retraction Note to multiple articles in Tumor Biology
Detection of circulating tumor cells (CTCs) has been made to develop reliable assays for early diagnosis of various cancers. Overexpression of survivin in cancer cells is strongly associated with tumor progression. Although upregulation of survivin is observed in various tumors, its expression profile in the peripheral blood of prostate cancer (PCa) patients has not yet been investigated. In this study, we validated the application of survivin as the tumor marker to detect CTC and assessed its utility for diagnosis of PCa distant metastasis. Immunohistochemistry and quantitative real-time PCR (QRT-PCR) were performed to confirm the levels of surviving expression in PCa tissues. In addition, CTC values in 3 mL of peripheral blood from PCa patients, benign prostate hyperplasia (BPH) patients, and normal controls were also measured by the survivin-targeted PCR. Our results showed that surviving was overexpressed in PCa tissues. The median levels of blood surviving mRNA of PCa patients, BPH patients, and normal controls were 5.67 (range from 0 to 12.46), 2.24 (range from 0 to 6.55), and 1.85 (range from 0 to 3.82), respectively. The levels of survivin are positively associated with PCa distant metastasis. Our results concluded that quantitation of CTCs through survivin-PCR could be a promising marker for diagnosis of PCa metastasis.