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4116831 
Journal Article 
Stability indicating high performance liquid chromatography methods for 5-fluorouridine in aqueous solution 
Dorta, MJ; Munguia, O; Farina, JB; Martin, VS; Llabres, M 
1997 
1 
Arzneimittel-Forschung
ISSN: 0004-4172
EISSN: 1616-7066 
47 
12 
1388-1392 
WOS:000071834400009 
Two methods of reversed-phase high performance liquid chromatography (RP-HPLC) were used to detect and quantify the degradation of 5-fluorouridine (GAS 316-46-1, 5-FUR) in aqueous solution. The H-1-NMR technique was used for indentifying the degradation products. Several assays were performed from different aqueous solutions of 5-FUR. The best results (optimal resolution, reproducibility and rapidity) were achieved by using Resolve(R) C-18 as stationary phase and a mix (96:4) of 50 mmol/l ammonium dihydrogen phosphate (adjusted to pH 3.5 with phosphoric acid) and acetonitrile as mobile phase. Despite the 5-FUR hydrolysis to 5-fluorouracil (5-FU) and D-ribose under moderate conditions in unlikely due to high stability of the nucleoside, these were the identified degradation products in assays carried out. 
CAS 316-46-1; 5-fluorouridine, degradation, HPLC methods