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4118236 
Journal Article 
HPLC determination of chlorhexidine gluconate and p-chloroaniline in topical ointment 
Havlíková, L; Matysová, L; Nováková, L; Hájková, R; Solich, P 
2007 
Yes 
Journal of Pharmaceutical and Biomedical Analysis
ISSN: 0731-7085
EISSN: 1873-264X 
43 
3 
1169-1173 
English 
17097258 
WOS:000244623000053 
A novel fast isocratic reversed-phase HPLC method for simultaneous determination of chlorhexidine and its degradation product p-chloroaniline was developed. Zorbax SB Phenyl column (75 mm x 4.6 mm, 3.5 microm) was used for the separation. Mobile phase composed of acetonitrile and buffer solution of 0.08 M sodium phosphate monobasic containing 5 ml of triethylamine (0.5%) and adjust with 85% phosphoric acid to pH 3.0 in ratio 35:65 (v/v) pumped isocratically at flow rate 0.6 ml min(-1) was used. UV detection was performed at 239 nm, the total analysis time was about 10 min. The method is suitable for practical routine analysis of topical ointment in the quality control laboratory. 
Chlorhexidine; HPLC; Ointment