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4120184 
Technical Report 
Determination of sodium fluoroacetate (Compound 1080) in poison baits by HPLC 
Collins, DM; Fawcett, JP; Rammell, CG 
1981 
PESTAB/81/2670 
Environ 
5 
PESTAB. A technique for the determination of sodium fluoroacetate (Compound 1080) in poison baits for vertebrate pests is described. A 20-gram sample is homogenized in 200 ml of water, then brought to volume (100 ml) and left overnight. A 1.0-ml sample is mixed with 100 mul 5 M orthophosphoric acid and distilled; pH adjusted and then sample is derivatized. The samples are chromatographed on a 25-cm RP-8 reverse phase column. A high pressure liquid chromatography system is used, comprising of a Tracor 995 pump, a Rheodyne 7120 injector with 100-mul loop, and a Varian Fluorichrom fluorescence detector. Recoveries of 83 and 99% were obtained for duplicate samples of bait spiked with 100 and 1000 mg of the pesticide.