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4120263 
Journal Article 
Determination Of Quinine In Hair Preparations By Reversed-Phase High-Performance Liquid Chromatography 
Cavazzutti, C; Gagliardi, L; Amato, A; Gattavecchia, E 
1983 
1 
Journal of Chromatography
ISSN: 0021-9673 
NIOSH/00141620 
257 
1 
166-169 
A reversed phase high performance liquid chromatography (HPLC) method was developed for determining quinine (130950) in hair preparations. A liquid chromatograph equipped with a variable wavelength ultraviolet detector was used. The analytical column was 250 millimeters by 4.6 millimeters containing silica. Chromatographic conditions were: mobile phase, 0.1 molar (M) phosphoric-acid, 0.1M potassium-dihydrogen-phosphate, 0.1M tetramethylammonium bromide, water, acetonitrile (10/50/100/340/88), pH 2.4; flow rate, 1 milliliter per minute; pressure, 900 pounds per square inch; column temperature, 25 degrees-C; injection volume, 10 microliters; detector wavelength, 332 nanometers. Amounts of quinine from 0.2 to 1.0 micrograms were determined. Recovery experiments were carried out by adding known amounts of quinine to four commercial products not containing quinine. Recoveries from quinine containing shampoo products were also assayed. Under the chromatographic conditions used, the retention time of quinine was 7.9 minutes and was reproducible, even over several days. The calibration graph obtained was linear up to 1 microgram quinine injected. The detection limit was approximately 5 nanograms calculated on a response of twice the noise value. Recovery of added quinine ranged from 97 to 99 percent of the theoretical content of the shampoos and lotions. Recovery of quinine containing products ranged from 97 to 104 percent. The authors conclude that the HPLC method is suitable for analyzing hair preparations containing quinine.