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4120958 
Journal Article 
The Prostaglandin H Synthase-Dependent Activation Of 2-Aminofluorene To Products Mutagenic To S. Typhimurium Strains TA98 And TA98NR 
Boyd, JA; Zeiger, E; Eling, TE 
1985 
1 
Mutation Research
ISSN: 0027-5107
EISSN: 1873-135X 
NIOSH/00151888 
143 
3 
187-190 
The mutagenic effects of prostaglandin-H synthase (PHS) activated 2-aminofluorene (153786) (AF) were investigated in Salmonella-typhimurium strains TA-98 and TA-98NR. These two bacteria strains were kept frozen at minus 80 degrees-C and then cultures were grown in an oxoid nutrient broth at 37 degrees overnight. Reaction mixtures were comprised of 0.5 milliliter ram seminal vesicle microsomes, diluted to 4 milligrams protein per milliliter in a 60 millimolar solution of potassium-chloride with 100 millimolar solution sodium-phosphate buffer, pH 7.4; 0.1 milliliter bacterial culture; and 0.05 milliliter dimethyl-sulfoxide. A comparison was made between the ability of a rat liver preparation and the PHS activity of ram seminal vesicle microsomes to activate AF. The activation of AF by PHS produced the same number of revertants in both bacteria strains. The PHS dependent activation of AF resulted in virtually identical dose response curves with both strains. The number of TA-98 revertants produced by the rat liver activation was roughly 1 order of magnitude greater than that produced by PHS activation. The authors conclude that the rat liver preparation mediates a greater mutagenic response to AF than the PHS system. An AF free radical species is responsible for PHS mediated mutagenicity.