Health & Environmental Research Online (HERO)


Print Feedback Export to File
4169815 
Journal Article 
Scaffolds based on chitosan/pectin thermosensitive hydrogels containing gold nanoparticles 
Tentor, FR; de Oliveira, JH; Scariot, DB; Lazarin-Bidóia, D; Bonafé, EG; Nakamura, CV; Venter, SAS; Monteiro, JP; Muniz, EC; Martins, AF 
2017 
Yes 
International Journal of Biological Macromolecules
ISSN: 0141-8130
EISSN: 1879-0003 
102 
1186-1194 
English 
28487197 
WOS:000406984300130 
Thermosensitive hydrogels based on chitosan/pectin (CS/Pec) and CS/Pec/gold nanoparticles (CS/Pec/AuNPs) were successfully prepared with different AuNP levels. Using a tilting method, gelation temperature was demonstrated to decrease when the amount of AuNPs increased and pectin concentrations decreased. The presence of AuNPs in the CS/Pec composite was evaluated via WAXS and UV-vis techniques, while SEM analysis assessed the average size of pores (350-600μm). All samples were extremely cytocompatible with many cell types, such as normal kidney epithelial cells (VERO cells), epithelial colorectal adenocarcinoma cells (HT-29 cells), HPV-16 positive human cervical tumour cells (SiHa cells), kidney epithelial cells (LLCMK2 cells) and murine macrophage cells (J774A1 cells). Cell viability assays using the MTT method upon mouse preosteoblastic cells (MC3T3-E1 cells) showed that CS/Pec and CS/Pec/AuNPs composites had the potential to foster proliferation and growth of bone cells, making them possible stimulators for reconstruction of bone tissues.