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4197119 
Journal Article 
Ultraviolet germicidal coil cleaning: Decreased surface microbial loading and resuspension of cell clusters 
Luongo, JC; Miller, SL 
2016 
Building and Environment
ISSN: 0360-1323
EISSN: 1873-684X 
105 
50-55 
Cooling coil surfaces within building ventilation systems are ideal sites for biofilm formation due to the presence of adequate nutrients (deposited particles) and moisture (condensate). In this study, a heating, ventilation, and air-conditioning (HVAC) test apparatus was built consisting of two parallel ducts, each with its own cooling coil. One coil was exposed to ultraviolet germicidal coil cleaning (UVG-CC) while the other was the comparison control to investigate the impact of UVG-CC on surface microbial loading and bacterial attachment. Surface samples were collected by swabbing a uniform area of coil surface and airborne samples were collected isokinetically with sterile funnel filters. All samples were quantified via direct epifluorescent microscopy. Prior to irradiating, higher concentrations of surface microbial loading were found on the downstream side of both cooling coils under condensing conditions. Conversely, under dry surface conditions with downstream UV irradiance, surface concentrations were higher upstream. UVG-CC (at an average 200 uW/cm(2) on the coil surface) reduced surface microbial loading by 55% on average during condensing conditions and inhibited bacterial attachment causing clusters of bacterial matter to become airborne downstream of the cooling coil. Additionally, it was found that desiccation also inhibited surface microbial loading and yielded cluster detachment but to a lesser degree than UVG-CC treatment. (C) 2016 Elsevier Ltd. All rights reserved. 
UVC; UVGI; Coil fouling; Heat exchanger; HVAC; Biofilm