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HERO ID
4218492
Reference Type
Journal Article
Title
Kinetic analysis of the interaction between poly(amidoamine) dendrimers and model lipid membranes
Author(s)
Tiriveedhi, V; Kitchens, KM; Nevels, KJ; Ghandehari, H; Butko, P
Year
2011
Is Peer Reviewed?
1
Journal
Biochimica et Biophysica Acta
ISSN:
0006-3002
EISSN:
1878-2434
Publisher
ELSEVIER
Location
AMSTERDAM
Volume
1808
Issue
1
Page Numbers
209-218
Language
English
PMID
20828535
DOI
10.1016/j.bbamem.2010.08.017
Web of Science Id
WOS:000285853800022
Abstract
We used fluorescence spectroscopy and surface tensiometry to study the interaction between low-generation (G1 and G4) poly(amidoamine) (PAMAM) dendrimers, potential vehicles for intracellular drug delivery, and model lipid bilayers. Membrane association of fluorescently labeled dendrimers, measured by fluorescence anisotropy, increased with increasing size of the dendrimer and with increasing negative charge density in the membrane, indicating the electrostatic nature of the interaction. When the membrane was doped with pyrene-labeled phosphatidyl glycerol (pyrene-PG), pyrene excimer fluorescence demonstrated a dendrimer-induced selective aggregation of negatively charged lipids when the membrane was in the liquid crystalline state. A nonlinear Stern-Volmer quenching of dendrimer fluorescence with cobalt bromide suggested a dendrimer-induced aggregation of lipid vesicles, which increased with the dendrimer's generation number. Surface tensiometry measurements showed that dendrimers penetrated into the lipid monolayer only at subphysiologic surface pressures (<30mN/m). We conclude that the low-generation PAMAM dendrimers associate with lipid membranes predominantly electrostatically, without significantly compromising the bilayer integrity. They bind stronger to membranes with higher fluidity and lower surface pressure, which are characteristic of rapidly dividing cells.
Keywords
Cell-penetrating molecules; Fluorescence spectroscopy; Membrane fluidity; PAMAM dendrimers; Quenching; Resonance energy transfer
Tags
IRIS
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Cobalt
LitSearch: January 2008 - August 2018
PubMed
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